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KMID : 0644120190320030020
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Journal of Oriental Gynecology
2019 Volume.32 No. 3 p.20 ~ p.31
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Anti-Inflammatory Effect of Ethanol Extract from the seeds of Arctium Lappa L. in Vascular Endothelial Cells
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Lee Yun-Jung
Yoon Jung-Joo
Kim Hye-Yoom
Ahn You-Mee
Hong Mi-Hyeon
Son Chan-Ok
Na Se-Won
Lee Ho-Sub
Kang Dae-Gill
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Abstract
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Objectives: The seeds from Arctium lappa have been considered for its various pharmacological properties, which include anti-carcinogenic, anti-inflammatory, anti-diabetic, and anti-viral activities.
Methods: In the present study, we investigated the anti-inflammatory effect of the ethanol extract from the seeds of Arctium lappa L (EAL) on cytokine-induced vascular inflammation in human umbilical vein endothelial cells (HUVEC).
Results: Pretreatment with EAL significantly decreased TNF-α-induced cell adhesion molecules expression such as ICAM-1, VCAM-1, and E-selectin in a dose-dependent manner. Cell adhesion assay showed that pretreatment with EAL suppressed HUVEC-monocyte adhesion by TNF-α over 1 μg/ml concentration. We investigated the involvement of nuclear transcription factor kappa-B (NF-κB) in TNF-α-induced vascular inflammation. NF-κB p65 nuclear expression was induced by TNF-α, however, pretreatment with EAL was attenuated that nuclear translocation. In cytoplasm, EAL was also attenuated TNF-α-induced decrease of inhibitor of κB-α (IκB-α) expression. Moreover, EAL significantly decreased TNF-α-induced production of intracellular reactive oxygen species (ROS).
Conclusions: Taken together, our findings suggest that seeds of Arctium lappa L could be a therapeutic herb for prevention of cardiovascular diseases throughout the inhibition of vascular endothelial inflammation.
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KEYWORD
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Arctium lappa L, vascular inflammation, cardiovascular diseases, cell adhesion molecules, NF-κB
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